In Cellulo Mössbauer and EPR Studies Bring New Evidence to the Long‐Standing Debate on Iron–Sulfur Cluster Binding in Human Anamorsin

Human anamorsin is an iron–sulfur (Fe–S)-cluster-binding protein acting as electron donor in the early steps of cytosolic biogenesis. belongs to eukaryotic CIAPIN1 family and contains two highly conserved cysteine-rich motifs, each binding Fe–S cluster. In vitro works by various groups have provided rather controversial results for type clusters bound proteins. order unravel knot on this topic, we used cellulo approach combining Mössbauer EPR spectroscopies characterize iron–sulfur-cluster-bound form human anamorsin. We found that binds [2Fe–2S] at both its motifs.